Dr. Charles Runels Explains Science Behind Popular PRP Procedures for Female Genitalia

New clinical data presented by Charles Runels, MD, founder of the Cellular Medicine Association (CMA), reveal sustained improvements in female sexual function 1 year after treatment with the O-Shot®, a platelet-rich plasma (PRP) protocol for vaginal and clitoral injection.

Dr. Runels shared the findings during the European Society of Aesthetic Gynecology's 9th World Congress in Marrakesh, Morocco, where he discussed outcomes from a large clinical cohort and emerging combination approaches to enhance genital vascularity and neural signaling. The presentation underscored the evolving scientific framework behind a procedure that has recently gained viral attention as the “Revenge Vagina,” following a fictional portrayal in Hulu’s All’s Fair.

While the term originates from pop culture, the data reflect a real and actively studied medical intervention. The O-Shot® protocol, first described in peer-reviewed literature in 2014, involves autologous PRP injected into the female genitalia to improve sexual function and address symptoms such as vaginal dryness, diminished arousal, and in some cases, urinary incontinence.

In Morocco, Dr. Runels presented sustained 1-year benefits in sexual function metrics based on patient-reported outcomes from a sizable clinical cohort. In addition to long-term efficacy, the lecture highlighted preliminary research on synergistic strategies that combine PRP with therapies targeting blood flow and neurogenesis—an area of growing interest in regenerative gynecology.

“The O-Shot® is not simply an injection—it’s a defined, evidence-based protocol supported by more than a decade of research and clinical use,” said Dr. Runels. “What we’re seeing now is an opportunity to build on that foundation with adjunctive therapies that may further improve outcomes.”

According to the Cellular Medicine Association, use of the O-Shot® name is restricted to licensed physicians trained in the standardized protocol (USPTO Serial No. 90975954). An educational explainer has been released by the CMA to clarify how media references relate to medical reality and to distinguish the protocol from unregulated imitators.